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Korean Journal of Preventive Medicine 2002;35(4): 269-274.
Effect of Glutathione on Lead Induced Modulation of NO Synthesis in RAW 264.7 Cell.
Sae Ron Shin, Gyung Jae Oh, Keun Sang Kwon, Wook Hee Yoon
1Department of Preventive Medicine and Public Health, Chonbuk National University Medical School and Institute for Medical Sciences, Korea. shinsr0704@hanmail.net
2Department of Preventive Medicine and Public Health, Wonkwang University Medical School, Korea.
ABSTRACT
OBJECTIVES: To evaluate the effect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages. METHODS: This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine(BSO). RESUJLTS: ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead(PbCl2) concentration 0.5 micro M. The presence of 300 micro M NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of 300 micro M BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used. CONCLUSIONS: These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)
Key words: Glutathione; Lead; Buthionine sulfoximine; N-acetylcysteine; Nitric oxide
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