1Center for Diagnostic Nanosystems, Marshall University, Huntington, WV, USA
2School of Kinesiology, College of Health Professions, Marshall University, Huntington, WV, USA
3Biotechnology Department, West Virginia State University, Institute, WV, USA
4Department of Internal Medicine, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USA
5Department of Pharmacology, Physiology and Toxicology, Marshall University, Joan C. Edwards School of Medicine, Huntington, WV, USA
6Health Effects Laboratory Division, NIOSH, Morgantown, WV, USA
7Department of Pharmaceutical Sciences, Marshall University School of Pharmacy, Huntington, WV, USA
Copyright © 2015 The Korean Society for Preventive Medicine
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
CONFLICT OF INTEREST
The authors have no conflicts of interest with the material presented in this paper.
Exposure (d) |
Body weight (g) |
Lung weight (g) |
||
---|---|---|---|---|
Saline control | CeO2 (7.0 mg/kg) | Saline control | CeO2 (7.0 mg/kg) | |
1 | 319.67±15.92 | 319.67±15.20 | 1.74±0.28 | 1.88±0.08 |
3 | 310.33±28.10 | 331.67±24.00 | 1.54±0.27 | 2.19±0.15 |
14 | 345.67±27.11 | 332.33±21.07 | 1.90±0.31 | 2.12±0.23 |
28 | 411.33±29.21,3 | 403.67±28.941,3 | 1.82±0.09 | 2.43±0.30 |
56 | 451.67±26.211-3 | 451.00±34.61-4 | 1.56±0.24 | 2.84±0.58 |
90 | 523.33±60.871-5 | 519.33±44.841-5 | 1.62±0.11 | 2.75±0.51 |
Lung tissues were collected from groups of animals that were intratracheally instilled with either normal saline or CeO2 nanoparticles at a dosage of 7.0 mg/kg, at 1, 3, 14, 28, 56, and 90 days after exposure. The lung tissues were cleaned to remove blood and tissue debris with Krebs’s lactate solution and weighed immediately (n=6/group). Two-way analysis of variance was performed for overall comparisons, while the Student-Newman-Keuls post hoc test was used to determine differences between groups; p<0.05 were considered to indicate statistical significance.
1 Significantly different from the one-day exposure group for a given condition.
2Significantly different from the three-day exposure group for a given condition.
3 Significantly different from the 14-day exposure group for a given condition.
4 Significantly different from the 28-day exposure group.
5 Significantly different from the 56-day exposure group.
Exposure (d) | Body weight (g) |
Lung weight (g) |
||
---|---|---|---|---|
Saline control | CeO2 (7.0 mg/kg) | Saline control | CeO2 (7.0 mg/kg) | |
1 | 319.67±15.92 | 319.67±15.20 | 1.74±0.28 | 1.88±0.08 |
3 | 310.33±28.10 | 331.67±24.00 | 1.54±0.27 | 2.19±0.15 |
14 | 345.67±27.11 | 332.33±21.07 | 1.90±0.31 | 2.12±0.23 |
28 | 411.33±29.2 |
403.67±28.94 |
1.82±0.09 | 2.43±0.30 |
56 | 451.67±26.21 |
451.00±34.6 |
1.56±0.24 | 2.84±0.58 |
90 | 523.33±60.87 |
519.33±44.84 |
1.62±0.11 | 2.75±0.51 |
Lung tissues were collected from groups of animals that were intratracheally instilled with either normal saline or CeO2 nanoparticles at a dosage of 7.0 mg/kg, at 1, 3, 14, 28, 56, and 90 days after exposure. The lung tissues were cleaned to remove blood and tissue debris with Krebs’s lactate solution and weighed immediately (n=6/group). Two-way analysis of variance was performed for overall comparisons, while the Student-Newman-Keuls post hoc test was used to determine differences between groups; Significantly different from the one-day exposure group for a given condition. 2Significantly different from the three-day exposure group for a given condition. Significantly different from the 14-day exposure group for a given condition. Significantly different from the 28-day exposure group. Significantly different from the 56-day exposure group.