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J Prev Med Public Health > Volume 40(1); 2007 > Article
Journal of Preventive Medicine and Public Health 2007;40(1): 16-22. doi: https://doi.org/10.3961/jpmph.2007.40.1.16
Increased DNA Damage of Lymphocytes in Korean Male Smokers.
Joohyun Lee, Eunil Lee, Eunha Oh, Juneyoung Lee, Donggeun Sul, Jooja Kim
1Department of Preventive Medicine, College of Medicine and Postgraduate Studies of Public Health, Graduate School, Korea University, Korea. eunil@korea.ac.kr
2Medical Research Center for Environmental Toxico-Genomics and Proteomics, College of Medicine, Korea University, Korea.
3Brain Korea21 Program for Biomedical Science, College of Medicine, Korea University, Korea.
4Department of Biostatistics, College of Medicine, Korea University, Korea.
5Graduate School, College of Medicine, Korea University, Korea.
6Department of Occupational Medicine, Soonchunhyang University Hospital, Korea.
ABSTRACT
OBJECTIVE: The purpose of this study was to evaluate the levels of DNA damage in human lymphocytes caused by smoking and other lifestyle factors. METHODS: The study population consisted of 173 normal healthy male adults from 21 to 59 years old. The demographic and lifestyle variables were obtained from administered questionnaires. The level of lymphocytic DNA damage in the peripheral blood was evaluated by the Comet assay. Statistical analyses were done by general linear model analysis and Dunnett's multiple comparison. RESULTS: The difference in DNA damage between smokers and non-smokers was statistically significant. The means for the Tail%DNA were found to be 10.48 in the current smokers and 9.60 in the non-smokers (p<0.05). The tail moment means were 1.58 and 1.45 (p<0.05) for the current smokers and non-smokers, respectively. The number of cigarettes smoked per day did not result in a significant difference in the level of DNA damage among the smokers. Other lifestyle factors such as age, and drinking and exercise habits were not related to DNA damage. CONCLUSIONS: The DNA damage in the lymphocytes of smokers was found to be significantly higher than that for non-smokers. However, the number of cigarettes smoked per day was not related to DNA damage. Further study is needed to evaluate the relationship between the amount of smoking and level of damage to DNA. In addition, the status of DNA repair activities should be assessed.
Key words: Comet assay; DNA damage; Lymphocytes; Life style; Smoking
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